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Volume 7 (2010 / 2011): Article 13
Procedures for extraction and purification of leaf wax biomarkers from peats.
by J.E. Nicholse
Published online: 31.12.2011
Palaeoecological and palaeoclimate reconstruction, using leaf wax biomarkers, is a relatively new subdiscipline of peatland science. The ability to process large numbers of samples rapidly for biomarkers makes this type of analysis particularly appealing. This review is a guide to the preparation of leaf waxes for analysis by gas chromatography. The main phases of preparation are extraction of soluble organic compounds from sediment, separation of the total extract into fractions of differing polarity, and the derivatisation of polar functional groups. The procedures described here are not meant be exhaustive of all organic geochemical possibilities in peatlands, but a distillation of methods for the preparation of leaf waxes that are commonly and increasingly being used in palaeoecological and palaeoclimatological studies.
Nichols, J.E. (2011): Procedures for extraction and purification of leaf wax biomarkers from peats. Mires and Peat 7: Art. 13. (Online: http://www.mires-and-peat.net/pages/volumes/map07/map0713.php)
IMCG and IPS acknowledge the work of the reviewers.